Homologous Recombination: A GRAS Yeast Genome Editing Tool
نویسندگان
چکیده
منابع مشابه
Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination
Cas9 is an RNA-guided double-stranded DNA nuclease that participates in clustered regularly interspaced short palindromic repeats (CRISPR)-mediated adaptive immunity in prokaryotes. CRISPR-Cas9 has recently been used to generate insertion and deletion mutations in Caenorhabditis elegans, but not to create tailored changes (knock-ins). We show that the CRISPR-CRISPR-associated (Cas) system can b...
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The rate limiting step in translating normal and pathological human genetic variations into a deeper understanding of which are functionally relevant, and the development of genetically defined disease models that can speed up and rationalize the discovery of novel targeted therapies, now lies firmly with our ability to quickly and precisely alter any sequence in the human genome, just as we ha...
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Gene targeting in mammalian cells has proven invaluable in biotechnology, in studies of gene structure and function, and in understanding chromosome dynamics. It also offers a potential tool for gene-therapeutic applications. Two limitations constrain the current technology: the low rate of homologous recombination in mammalian cells and the high rate of random (nontargeted) integration of the ...
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Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
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ژورنال
عنوان ژورنال: Fermentation
سال: 2020
ISSN: 2311-5637
DOI: 10.3390/fermentation6020057